Isolation of Local Lipolytic Isolate from Domestic Compost

Screening of lipolytic bacteria from domestic compost resulting an isolate namely AL17. Morphological analysis shows that the isolates were rod shape and belong to negative gram bacteria. The 16S rRNAs genes of the bacteria have been sequenced, and phylogenetic analysis showed that the isolates were close to genus Pseudoxanthomonas. The enzyme production was synchronized with bacterial growth and reached a maximum level during the late-stationary phase. The optimum pH and temperature of enzyme activity were at pH 9.0 and 60°C.The isolate also showed alcohol tolerance in medium containing 3% and 5% methanol. The ability of bacterial cells to tolerate methanol is an important cell characteristic that determines their use as a biocatalyst in transesterification and other industrial process

Novel Archaeal DNA Polymerase B from Domas Hot Spring West Java

Nine novel archaeal DNA polymerase genes from Domas Hot Spring, West Java have been cloned directly through the natural sample. The characterization of the genes showed that the genes are high homology to the DNA polymerase B of Crenarhaea phyla. Phylogenetic analysis of the amino acid sequences showed that the enzymes are grouped in a new branch from the other Crenarchaea’s DNA Polymerase B. 3D structure analysis of the enzymes show that the structures are closed to the structure of DNA Polymerase B1 from Sulfolobus solfataricus. The nine structures of the enzymes could be grouped into four different structures

Ribotyping Identification of Thermophilic Bacterium from Papandayan Crater

A few thermophilic bacteria were isolated from a hot spring located in Papandayan Crater, Garut. One of the organisms showed a well growth at temperature of up to 80 oC. Chromosomal DNA from the organism was isolated and used to amplify 16S rRNA gene fragment. The gene was amplified by a set of universal primers (27F and 1492R) resulting in a 1.5 kb DNA fragment. The gene was cloned and sequenced. The phylogenetic tree, homological analysis, and detailed comparison of the sequences showed that 16S rRNA gene sequence of the Papandayan isolate is unique compared to other known strains, however the sequence had closest similarities with Bacillus caldolyticus and Bacillus caldotenax

The Role of Electrostatic Interactions on Klentaq1 Insight for Domain Separation

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Ribotyping Identification of Thermophilic Bacterium from Papandayan Crater

A few thermophilic bacteria were isolated from a hot spring located in Papandayan Crater, Garut. One of the organisms showed a well growth at temperature of up to 80oC. Chromosomal DNA from the organism was isolated and used to amplify 16S rRNA gene fragment. The gene was amplified by a set of universal primers (27F and 1492R) resulting in a 1.5 kb DNA fragment. The gene was cloned and sequenced. The phylogenetic tree, homological analysis, and detailed comparison of the sequences showed that 16S rRNA gene sequence of the Papandayan isolate is unique compared to other known strains, however the sequence had closest similarities with Bacillus caldolyticus and Bacillus caldotenax

Translation Initiation Factors eIF3 and HCR1 Control Translation Termination and Stop Codon Read-Through in Yeast Cells

Translation is divided into initiation, elongation, termination and ribosome recycling. Earlier work implicated several eukaryotic initiation factors (eIFs) in ribosomal recycling in vitro. Here, we uncover roles for HCR1 and eIF3 in translation termination in vivo. A substantial proportion of eIF3, HCR1 and eukaryotic release factor 3 (eRF3) but not eIF5 (a well-defined “initiation-specific” binding partner of eIF3) specifically co-sediments with 80S couples isolated from RNase-treated heavy polysomes in an eRF1-dependent manner, indicating the presence of eIF3 and HCR1 on terminating ribosomes. eIF3 and HCR1 also occur in ribosome- and RNA-free complexes with both eRFs and the recycling factor ABCE1/RLI1. Several eIF3 mutations reduce rates of stop codon read-through and genetically interact with mutant eRFs. In contrast, a slow growing deletion of hcr1 increases read-through and accumulates eRF3 in heavy polysomes in a manner suppressible by overexpressed ABCE1/RLI1. Based on these and other findings we propose that upon stop codon recognition, HCR1 promotes eRF3·GDP ejection from the post-termination complexes to allow binding of its interacting partner ABCE1/RLI1. Furthermore, the fact that high dosage of ABCE1/RLI1 fully suppresses the slow growth phenotype of hcr1? as well as its termination but not initiation defects implies that the termination function of HCR1 is more critical for optimal proliferation than its function in translation initiation. Based on these and other observations we suggest that the assignment of HCR1 as a bona fide eIF3 subunit should be reconsidered. Together our work characterizes novel roles of eIF3 and HCR1 in stop codon recognition, defining a communication bridge between the initiation and termination/recycling phases of translation